034 Tissue specificity of dendritic cells supersedes subset identity

To prevent acute life-threatening auto-inflammation/autoimmune of the tissues, our immune system must mount protective immunity, and rapidly resolve inflammation. Dendritic cells (DCs) are specialized sentinels that maintain homeostasis by efficiently regulating balance between immunity tolerance to self-antigens. It is critical understand how tissue DCs subsets their specificity governed mediate or tolerant immunity. By assaying chromatin accessibility LN resident (cDC1 vs cDC2) migratory (migDC1 migDC2) genome-wide through ATACseq analysis, together with prior lab work using RNAseq we found myeloid maturation migration programing supersedes subset diversification, enforced at level chromatin. No major differences in were detected on DC from CD11cCre-/IFNgR1flfl (WT) versus CD11cCre+/IFNgR1flfl (IFNgR1ΔDC) mice however, indicating IFNg/IFNgR1 signaling may function as a potential local, instead global instructive cue. IFNgR1 promotor analysis cDCs migDCs, confirms site-specific regulation leading protein level. Further studies will explore regulates manner identify tissue-specific transcription factors (TFs) controlling promoter homeostasis.